Phospho-stat3 tyr705
WebPE anti-STAT3 Phospho (Tyr705) Antibody. Human whole blood was stimulated with (top), or without (bottom), IL-6 for 15 minutes, and then treated with RBC Lysis/Fixation Solution (10X), surface stained with CD3 APC, permeabilized with True-Phos™ Perm Buffer, and then intracellularly stained with STAT3 Phospho (Tyr705) (clone 13A3-1) PE ... WebJun 2, 2024 · Mechanistically, phosphorylated STAT3 (Tyr705) inhibition resulting from pimozide treatment caused a downregulation of downstream transcriptional targets such as matrix metalloproteinase-9 (MMP-9) and vimentin, both implicated in …
Phospho-stat3 tyr705
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WebAlexa Fluor® 488 anti-STAT3 Phospho (Tyr705) Antibody. Human whole blood was stimulated with (top) or without (bottom) IL-6 for 15 minutes, then treated with RBC Lysis/Fixation Solution and permeabilized with True-Phos™ Perm Buffer, then stained with CD3 APC and STAT3 pY705 (clone 13A3-1) Alexa Fluor® 488. Compare all formats See … WebFeb 19, 2024 · Interestingly, the phosphorylation of STAT3 (Tyr705), which occurs downstream of JAK1/2, but not STAT3 (Ser727), which occurs downstream of ERK, was slightly decreased at 5–10 min and significantly downregulated at 15 min post-irradiation .
WebJul 8, 2024 · To genetically suppress phospho-Tyr705 of STAT3 (pY-STAT3), the Y705 phosphorylation STAT3 mutant (Y705A) in which Y705 was replaced with alanine was generated from the GV492 vector (GeneChem, Shanghai, China) containing Ubi-MCS-3FLAG-CBh-gcGFP-IRES-puromycin. The Raw264.7 cells were transfected following the … WebTest was conducted on a previous lot. Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 …
WebFeb 2, 2008 · The Tyr705 phosphorylation triggered by LIFRβ promotes STAT3 dimerization and translocation to the nucleus, where STAT3 regulates transcription of cytokine-responsive genes (Darnell et al. 1994). PTEN loss or expression of a constitutively active Akt in astrocytes significantly inhibited STAT3 phosphorylation concomitantly with LIFRβ … WebSTAT3 is activated by Tyr705 phosphorylation in response to membrane factors such as interleukin-6 (IL-6). 45 It has also been shown that Ser727 of STAT3 is phosphorylated by various kinds of kinases in various cancers. 46–49 However, some reports have shown that the Ser727 site phosphorylation suppresses STAT3 activity and cell proliferation ...
WebThe STAT3 (Tyr705)Phospho- Assay is a sandwich immunoassay (Figure 1). MSD provides a plate that has been pre-coated with capture antibody for STAT3total . The user adds the sample and a solution containing the detection antibody—anti-phospho-STAT3 (Tyr705) conjugated with an electrochemiluminescent
WebPhosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to … north african goodshow to renew working with children check vicWebApr 23, 2012 · In the STAT3 (Tyr705) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti-Phospho-STAT3 (Tyr705) or Anti-STAT3 is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-mouse IgG is added to the wells. how to renew wisconsin nursing licenseWebApr 12, 2024 · The effect of FLU on STAT3 expression and activation in A172 and T98G cells. (A) The time dependence of FLU effect on STAT3 and phospho-STAT3 (Tyr705) … how to renew work permit in chinaWebSTAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer … how to renew wisconsin drivers licenseWebFITC anti-STAT3 Phospho (Tyr705) Antibody. Human whole blood was stimulated with (left) or without (right) IL-6 for 15 minutes, then treated with RBC Lysis/Fixation Solution (Cat No. 422401), and permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with CD3 APC and STAT3 pY705 (clone 13A3-1) FITC. how to renew work permit singaporeWebAnti-STAT3 (phospho Tyr705) antibody [HL1464] See all STAT3 primary antibodies Description Rabbit monoclonal [HL1464] to STAT3 (phospho Y705) - BSA and Azide free Host species Rabbit Tested applications Suitable for: WB more details Species reactivity Reacts with: Rat, Human Immunogen Synthetic peptide. north african guitar music